Biocatalysis
About
We build complex products from renewable raw materials using synthetic enzyme cascades. For this purpose, we combine - in a modular and flexible way - enzymes that do not occur together in nature. Rational enzyme engineering, reaction optimization and process design are used to obtain economically and ecologically efficient cascades.
An interdisciplinary team of (bio)chemists, biologists and engineers develops these multi-enzyme catalyzed processes with high step and atom efficiency while avoiding (toxic) by-products.
Starting from low-cost material, especially high-value (optically active) fine chemicals, often with direct pharmaceutical application, but also building blocks for chemicals or biofuels are produced. So far, product platforms of 2-hydroxyketones, amines, amino alcohols, diols and tetrahydroisoquinolines (the latter showing e.g. great potential as antibiotics and anticancer drugs) have been established. Some processes are successfully scaled up with collaborative partners from industry.
Research Topics
Current and future research targets are
- the use of continuous systems and unconventional media to increase space-time-yields > read more
- combining enzymes, chemical catalysts and microbial cell factories to generate value from renewable resources > read more
- regulation of enzyme activities in cascades using light > read more
- data management in biocatalysis using FAIR principles > read more
Research activities take place at Forschungszentrum Jülich GmbH while teaching activities are conducted at RWTH Aachen University (Aachen Biology and Biotechnology) in the Teaching- and Research Area Synthetic Enzyme Cascades.
Members
Scientific staff
Technicians
PhD students
Student
News & Gallery
Publications
selected press releases & videos
"What can biotechnology do?" a talk as part of a seminar organized by the Aachen computer scientists for a non-specialist audience. January 17th 2024, Recording and publication on Youtube: https://www.youtube.com/watch?v=HVGxaAJFN6Y
Bioeconomy Blog. Im Takt der Temperatur: das Enzym Orchester I Falling Walls Rheinland
https://blogs.fz-juelich.de/biooekonomie/2020/11/16/im-takt-der-temperatur-das-enzym-orchester/
Mit Biotech die Welt retten. Geht das? Faktencheck Folge 1
Online-Wissensmagazin zur Bio-Innovation
Konzept: Futurium Berlin | Produktion: Februar Film | Moderation: Barbara Scherle
https://www.youtube.com/playlist?list=PL1lO4YK4FC3ht0p1L77nqKF6sjdNkUk03
https://www.facebook.com/futuriumD/videos/786937728749330/
Video "MyScience". RWTH Aachen University: Prof. Dörte Rother
https://www.youtube.com/watch?v=Nil-j8a218w
DECHEMA Price 2019. Press release
https://www.fz-juelich.de/de/aktuelles/news/pressemitteilungen/2019/2019-01-17-doerte-rother-erhaelt-den-dechema-preis-2018
Die Meisterin der Enzyme. Portrait in: Helmholtz Perspektiven. 01/2019: 40-42
https://www.helmholtz.de/newsroom/artikel/die-meisterin-der-enzyme/
10 Köpfe für unsere Zukunft – Nachwuchsforscher aus NRW. Bild online. 18.10.2017. http://www.bild.de/regional/ruhrgebiet/forschung/10-koepfe-fuer-unsere-zukunft-53561054.bild.html
Publications in peer-reviewed journals
Nicolas M, Küsters-Spöring J D, Aufderheide C, Traving H, Kipp C, Pfennig V, Bolm C, Siegert P, Rother D. 2025. Enzymatic asymmetric synthesis of all stereoisomers of aliphatic, vicinal diols in conventional and non‐conventional media. Advanced Synthesis & Catalysis, e202401143
Nicolas M, Gaelings N, Wiesenthal J, von Westarp W G, Pehlivanlar B, Pischinger S, Jupke A, Klankermayer J, Rother, D. 2025. Combination of Bio‐and Organometallic Catalysis for the Synthesis of Dioxolanes in Organic Solvents. ChemCatChem, e202401836.
Malzacher S, Meißner D, Range J, Blažević Z F, Rosenthal K, Woodley J M, Wohlgemuth R, Wied R, Nidetzky B, Giessmann R T, Prakinee K, Chaiyen P, Bommarius A S, Rohwer J M, de Souza R O M A, Halling P J, Pleiss J, Kettner C, Rother D. 2024. The STRENDA Biocatalysis Guidelines for cataloguing metadata. Nature Catalysis. 7(12), 1245-1249
Klos N, Osterthun O, Mengers H G, Lanzerath P, Graf von Westarp W, Lim G, Gausmann M, Küsters-Spöring J-D, Wiesenthal J, Guntermann N, Lauterbach L, Jupke A, Leitner W, Blank L M, Klankermayer J, Rother D. 2024. Concatenating Microbial, Enzymatic, and Organometallic Catalysis for Integrated Conversion of Renewable Carbon Sources. JACS Au. 4, (12), 4546–70
Lim G, Calabrese D, Wolder A, Cordero P R, Rother D, Mulks F F, Paul C E, Lauterbach, L. 2024. H2-driven biocatalysis for flavin-dependent ene-reduction in a continuous closed-loop flow system utilizing H2 from water electrolysis. Commun. Chem.7(1): 200
Rothkranz B, Rieb M, Unrau E L, Frindi-Wosch I, Hemmerich J, Sehl T, Rother D. 2024. High cell density cultivation combined with high specific enzyme activity: Cultivation protocol for the production of an amine transaminase from Bacillus megaterium in E. coli. ChemBioChem 25(9): e202400006
Graf von Westarp W, Wiesenthal J, Spöring J D, Mengers H G, Kasterke M, Koß H J, Blank L, Rother D, Klankermayer J, Jupke A. 2023. Interdisciplinary development of an overall process concept from glucose to 4, 5-dimethyl-1, 3-dioxolane via 2, 3-butanediol. Commun. Chem.6 (1): 253
Glaser S J, Pyo S H, Rehnberg N, Rother D, Hatti-Kaul R. 2023. Carboligation of 5-(hydroxymethyl)furfural via whole-cell catalysis to form C12 furan derivatives and their use for hydrazone formation. Microb. Cell Fact. 22: 120. https://doi.org/10.1186/s12934-023-02130-1
Weber D, de Souza Bastos L,Winkler M, Hailes H C, RotherD. 2023. Enzymatic cascades using purified and whole-cell biocatalysts towards a 1,3,4-substituted tetrahydoisoquinoline. RSC Advances 13 (15): 10097-10109
Lauterbach S, Dienhart H, Range J, Malzacher S, Spöring JD, Rother D, Pinto MF, Martins P, Lagerman CE, Bommarius AS, Høst AV, Woodley J, Ngubane S, Kudanga T, Bergmann F, Rohwer J, Iglezakis D, Weideman A, Wittig U, Swainston N, Kettner C, Schnell S, Pleiss J. 2023. EnzymeML: seamless data flow and modelling of enzymatic data. Nature Methods. 1-3.
Sehl T, Seibt L, Kappauf K, Ergezinger P, Spöring J-D, Mielke K, Graf von Westarp W, Verma N, Bocola M, Daussmann T, Chen H, Shi S, Jupke A, Rother D. 2023. Enzymatic (2R,4R)-pentanediol synthesis – “putting a bottle on the table”. Chem. Ing. Techn., cite.202200178.R1
Spöring J D, Wiesenthal J, Pfennig V S, Gätgens J, Beydoun K, Bolm C, Klankermayer J, Rother, D. 2023. Effective production of selected dioxolanes by sequential bio‐and chemocatalysis enabled by adapted solvent switching. ChemSusChem. 16 (2): e202201981
Mengers H G, Guntermann N, von Westarp W, Jupke A, Klankermayer J, Blank L M, Walter Leitner W, Rother D. 2023. Three Sides of the Same Coin: Combining Microbial, Enzymatic, and Organometallic Catalysis for Integrated Conversion of Renewable Carbon Sources. Chem. Ing. Techn., DOI: 10.1002/cite.202200169
Labib M, Grabowski L, Brüsseler C, Kallscheuer N, Wachtendonk L, Fuchs T, Jupke A, Wiechert W, Marienhagen J, Rother D, Noack S. 2022. Toward the Sustainable Production of the Active Pharmaceutical Ingredient Metaraminol. ACS Sus. Chem. Eng. 10 (16): 5117–5128
Spöring J-D, Graf von Westarp W, Kipp C R, Jupke A, Rother D. 2022. Enzymatic Cascade in a Simultaneous, One-Pot Approach with In Situ Product Separation for the Asymmetric Production of (4S,5S)-Octanediol. Org. Process Res. Dev. DOI: 10.1021/acs.oprd.1c00433
Gerlach T, Schain J, Söltl S, van Schie M M C H, Hilgers F, Bitzenhofer N L, Drepper T, Rother D. 2022. Photo-regulation of Enzyme Activity: The Inactivation of a Carboligase with Genetically Encoded Photosensitizer Fusion Tags, Frontiers Catal. https://doi.org/10.3389/fctls.2022.835919
Doeker M, Grabowski L, Rother D, Jupke A. 2022. In situ reactive extraction with oleic acid for process intensification in amine transaminase catalyzed reactions. Green Chem. 24 (1): 295-304
Oeggl R, Glaser J, von Lieres E, Rother D. 2021. Continuous enzymatic stirred tank reactor cascade with unconventional medium yielding high concentrations of (S)-2-hydroxyphenyl propanone and its derivatives. Catal. Sci. Technol. 11: 7886 - 7897
Siedentop R, Claaßen C, Rother D, Lütz S, Rosenthal K. 2021. Getting the Most Out of Enzyme Cascades: Strategies to Optimize In Vitro Multi-Enzymatic Reactions. Catalysts. 11 (10): 1183
Kappauf K, Majstorovic N, Agarwal S, Rother D, Claaßen C. 2021. Modulation of Transaminase Activity by Encapsulation in Temperature‐Sensitive Poly (N‐acryloyl glycinamide) Hydrogels. ChemBioChem 12 (4): 1190
Gerlach T, Nugroho D L, Rother D. 2021. The effect of visible light on the catalytic activity of PLP-dependent enzymes. ChemCatChem 13: 2398-2406
Mack K, Doeker M, Grabowski L, Jupke A, Rother D. 2021. Extractive in-situ product removal for the application of naturally produced L-alanine as amine donor in enzymatic metaraminol production. Green Chem. 23: 4892-4901
Weber D, Patsch D, Neumann A, Winkler M, Rother D. 2021. Production of the carboxylate reductase from Nocardia otitidiscaviarum in a soluble, active form for in vitro applications. ChemBioChem 22, 1823
Malzacher S, Rother D. 2021. Computer-aided enzymatic retrosynthesis. News&Views article. Nature Catal. 4.2: 92-93
Van Schie M M C H., Spöring J-D, Bocola M, Domínguez de María P, Rother D. 2021. Applied biocatalysis beyond just buffers – from aqueous to unconventional media. Options and guidelines. Green Chem. 23: 3191-3206
Claaßen C, Mack K, Rother D. 2020. Benchtop NMR for online reaction monitoring of the biocatalytic synthesis of aromatic amino alcohols. ChemCatChem. 12(4): 1190-119
Contente M L, Dall’Oglio F, Annunziata F, Molinari F, Rabuffetti M, Romano D, Tamborini L, Rother D, Pinto A. 2020. Stereoselective reduction of prochiral cyclic 1,3-diketones using different biocatalysts. Catalysis Letters 150 (4): 1176-1185
Erdmann V, Sehl T, Frindi-Wosch I, Simon R C, Kroutil W, Rother D. 2019. Methoxamine Synthesis in a Biocatalytic 1-Pot 2 Step Cascade Approach. ACS Catal. 9 (8): 7380-7388
Claaßen C, Gerlach T, Rother D. 2019. Stimulus-Responsive Regulation of Enzyme Activity for One-Step and Multi-Step Syntheses. Adv. Synth. Catal. 361(11): 2387–2401
Kulig J, Sehl T, Mackfeld U, Wiechert W, Pohl M, Rother D. 2019. An Enzymatic 2-Step Cofactor and Co-Product Recycling Cascade towards a Chiral 1,2-Diol. Part I: Cascade Design. Adv. Synth. Catal. 361(11): 2607-2615
Oeggl R, Neumann T, Gätgens J, Romano D, Noack S, Rother D. 2018. Citrate as Cost-efficient NADPH regenerating agent. Front. Bioeng. Biotechnol. 6 (196)
Oeggl R, Maßmann T, Jupke A, Rother D. 2018. Four Atom Efficient Enzyme Cascades for All 4-Methoxyphenyl-1,2-propanediol Isomers Including Product Crystallization Targeting High Product Concentrations and Excellent E-Factors. ACS Sustainable Chem. Eng. 6 (9): 11819–11826
Zhang W, Fernández-Fueyo E, Ni Y, van Schie M, Gacs J, Renerie R, Wever R, Mutti F, Rother D, Alcalde M, Hollmann F. 2018. Selective aerobic oxidation reactions using a combination of photocatalytic water oxidation and enzymatic oxyfunctionalisations. Nature Catal. 1 (1): 55-62
Döbber J, Gerlach T, Offermann H, Rother D, Pohl M. 2018. Closing the gap for efficient immobilization of biocatalysts in continuous processes: HaloTag TM fusion enzymes for a continuous enzymatic cascade towards a vicinal chiral diol. Green Chem. 20: 544 - 552
Erdmann V, Lichman B R, Zhao J, Simon R C, Kroutil W, Ward J M, Hailes H C, Rother D. 2017. Enzymatic and chemoenzymatic 3-step cascades for the synthesis of stereochemically complementary trisubstituted tetrahydroisoquinolines. Angew. Chem. Int. Ed. 56 (41): 12503-12507
Maugeri Z and Rother D. 2017. Reductive Amination of Ketones Catalyzed by Whole Cell Biocatalysts Containing Imine Reductases (IREDs). J. Biotechnol. 258: 167 - 170
Schmidt S, de Almeida T P, Rother D, Hollmann F. 2017. Towards environmentally acceptable synthesis of chiral a-hydroxy ketones via oxidase-lyase cascades. Green Chem. 19 (5): 1226-1229
Sehl T, Bock S, Marx L, Maugeri Z, Walter L, Westphal R, Vogel C, Menyes U, Erhardt M, Müller M, Pohl M, Rother D. 2017. Asymmetric synthesis of (S)-phenylacetylcarbinol–closing a gap in C–C bond formation. Green Chem. 19: 380-384
Wachtmeister J, Jakoblinnert A, Rother D. 2016. Stereoselective Two-Step Biocatalysis in Organic Solvent: Toward All Stereoisomers of a 1,2-Diol at High Product Concentrations. Org. Process Res. Dev. 20 (10): 1744–1753
Buchholz P C F, Vogel C, Reusch W, Pohl M, Rother D, Spieß A, Pleiss J. 2016. BioCatNet: a database system for the integration of enzyme sequences and biocatalytic experiments. ChemBioChem 17 (21): 2093–2098
Wachtmeister J, Rother D. 2016. Recent advances in whole cell biocatalysis techniques bridging from investigative to industrial scale. Curr. Opin. Biotechnol. 42: 169–177.
Maugeri Z and Rother D. 2016. Application of imine reductases (IREDs) in micro-aqueous reaction systems. Adv. Syn. Catal. 358 (17): 2745-2750
Beigi M, Gauchenova E, Walter L, Waltzer S, Bonina F, Stillger T, Rother D, Pohl M, Müller M. 2016. Regio- and Stereoselective Aliphatic-Aromatic Cross-Benzoin Reaction: Enzymatic Divergent Catalysis. Chem. Eur. J. 22 (39): 13000-14005
Wachtmeister J, Mennicken P, Hunold A, Rother D. 2016. Modularized Biocatalysis: Immobilization of whole cells for preparative applications in micro-aqueous organic solvents. ChemCatChem 8 (3): 607-614
García-Junceda E, Lavandera I, Rother D, Schrittwieser J H. 2015. (Chemo)enzymatic cascades – Nature’s synthetic strategy transferred to the laboratory. J. Mol. Cat. B: Enzym. 114: 1-6.
Sehl T, Maugeri Z, Rother D. 2015. Multi-step synthesis strategies towards 1,2-amino alcohols with special emphasis on phenylpropanolamines. J. Mol. Cat. B: Enzym. 114: 65-71.
Holec C, Sandkuhl D, Rother D, Kroutil W, Pietruszka J. 2015. Chemoenzymatic Synthesis towards the Active Agent Travoprost. ChemCatChem 7: 3125-3130
Westphal R, Vogel C, Schmitz C, Pleiss J, Müller M, Pohl M, Rother D. 2014. A Tailor-made Chimeric Thiamine Enzyme for the Direct Asymmetric Synthesis of (S)-Benzoins. Angew. Chem. Int. Ed. 53 (35): 9376-9379.
Ein maßgeschneidertes chimäres Thiamindiphosphat-abhängiges Enzym zur direkten asymmetrischen Synthese von (S)-Benzoinen. Angew. Chem. 126 (35): 9530-9533.
Erdmann V, Mackfeld U, Rother D, Jakoblinnert A. 2014. Enantioselective, continuous (R)- and (S)-2-butanol synthesis: achieving high space-time-yields with recombinant E. coli cells in a micro-aqueous, solvent-free reaction system. J. Biotechnol. 191: 106-112.
Jakoblinnert A, Rother D. 2014. A two-step biocatalytic cascade in micro-aqueous medium: using whole cells to obtain high concentrations of a vicinal diol. Green Chem. 16: 3472-3482.
Sehl T, Hailes H C, Ward J M, Menyes U, Pohl M, Rother D. 2014. Efficient two-step biocatalytic strategies for the synthesis of all nor(pseudo)ephedrine isomers. Green Chem. 16: 3341-3348.
Wachtmeister J, Jakoblinnert A, Kulig J, Offermann H, Rother D. 2014. Whole Cell Teabag Catalysis for the Modularisation of Synthetic Enzyme Cascades in Micro-Aqueous Systems. ChemCatChem. 6 (4): 1051-1058.
Man H, Kedziora K, Kulig J, Frank A, Lavandera-García I, Gotor-Fernández V, Rother D, Hart S, Turkenburg J P, Grogan G. 2014. Structures of alcohol dehydrogenases from Ralstonia and Sphingobium spp. reveal the molecular basis for their recognition of ‘bulky-bulky’ ketones. Top. Catal. 57:356-365.
Baraibar A G, von Lieres E, Wiechert W, Pohl M, Rother D. 2014. Effective (S)-α-hydroxy ketone production: a reaction engineering approach. Top. Catal. 57: 401-411
Sehl T, Hailes H C, Ward J M, Wardenga R, von Lieres E, Offermann H, Westphal R, Pohl M, Rother D. 2013. Two Steps in One Pot: Enzyme Cascade for the Synthesis of Nor(pseudo)ephedrine from Inexpensive Starting Materials. Angew. Chem. Int. Ed. 52 (26):6772-6775.
Zwei Schritte in einem Reaktionsgefäß: Enzymkaskaden zur selektiven Synthese von Nor(pseudo)ephedrin aus kostengünstigen Ausgangsmaterialien. Angew. Chem. 125 (26): 6904-6908.
Hailes H, Rother D, Müller M, Westphal R, Ward J M, Pleiss J, Pohl M. 2013. Engineering Stereoselectivity of ThDP-dependent Enzymes. FEBS J. 280 (24): 6374-6394.
Baraibar A G, von Lieres E, Wiechert W, Pohl M, Rother D. 2013. Effective (S)-α-hydroxy ketone production: a reaction engineering approach. Top. Catal. 57: 401-411
Westphal R, Jansen S, Vogel C, Pleiss J, Müller M, Rother D, Pohl P. 2013. MenD from Bacillus subtilis: A potent catalyst for the enantiocomplementary asymmetric synthesis of functionalized α-hydroxy ketones. ChemCatChem. 6 (4): 1082–1088.
Man H, Kedziora K, Kulig J, Frank A, Lavandera-García I, Gotor-Fernández V, Rother D, Hart S, Turkenburg J P, Grogan G. 2013. Structures of alcohol dehydrogenases from Ralstonia and Sphingobium spp. reveal the molecular basis for their recognition of ‘bulky-bulky’ ketones. Top. Catal. 57:356-365.
Westphal R, Hahn D, Mackfeld U, Waltzer S, Beigi M, Widmann M, Vogel C, Pleiss J, Müller M, Rother D, Pohl P. 2013. Tailoring (S)-selectivity of MenD from Escherichia coli. ChemCatChem. 5 (12): 3587-3594.
Westphal R, Waltzer S, Mackfeld U, Widmann M, Pleiss J, Beigi M, Müller M, Rother D, Pohl P. 2013. (S)-selective MenD variants from Escherichia coli provide access to new functionalized chiral α-hydroxy ketones. Chem. Commun. 49: 2061-2063.
Kulig J, Frese A, Kroutil W, Pohl M, Rother D. 2013. Biochemical characterization of an alcohol dehydrogenase from Ralstonia sp. Biotech. Bioeng. 110 (7): 1838-1848.
Sehl T, Simon R C, Hailes H C, Ward J M, Schell U, Pohl M, Rother D. 2012. TTC-based screening assay for ɷ-transaminases: a rapid method to detect reduction of 2-hydroxy ketones. J. Biotechnol., 159: 188-194.
Kulig J, Simon R C, Rose C A, Husain S M, Häckh M, Lüdeke S, Zeitler K, Kroutil W, Pohl M, Rother D. 2012. Stereoselective synthesis of bulky 1,2-diols with alcohol dehydrogenases. Catal. Sci. Technol., 2: 1580-1589.
Gerhards T, Mackfeld U, Bocola M, von Lieres E, Wiechert W, Pohl M, Rother D. 2012. Influence of organic solvents on enzymatic asymmetric carboligations. Adv. Synth. Catal., 354: 2805-2820.
Rother D, Kolter G, Gerhards T, Berthold CL, Gauchenova E, Knoll M, Pleiss J, Müller M, Schneider G, Pohl M. 2011. (S)-selective mixed benzoin condensation by structure-based design of the pyruvate decarboxylase from Acetobacter pasteurianus. ChemCatChem 3 (10): 1587-1596.
Khatri NK, Gocke D, Trentmann O, Neubauer P, Hoffmann F. 2011. Single-chain antibody fragment production in Pichia pastoris: Benefits of prolonged pre-induction glycerol feeding. Biotechnol. J. 6 (4): 452-462.
Gocke D, Kolter G, Müller M, Pohl M. 2010. Entwicklung einer Enzym-Plattform für die biokatalytische C-C Verknüpfung. Chem. Ing. Tech. 82: 81-86.
Gocke D, Graf T, Brosi H, Frindi-Wosch I, Walter L, Müller M, Pohl M. Comparative characterisation of thiamin diphosphate-dependent decarboxylases. 2009. J. Mol. Catal. B: Enzym. 61: 30-35.
Müller M, Gocke D, Pohl M. 2009. Thiamin diphosphate in biological chemistry: Exploitation of ThDP-dependent enzymes for asymmetric chemoenzymatic synthesis. FEBS J. 276 (11): 2894-2940.
Gocke D, Walter L, Gauchenova E, Kolter G, Knoll M, Berthold CL, Schneider G, Pleiss J, Müller M, Pohl M. 2008. Rational protein design of ThDP-dependent enzymes: engineering stereoselectivity. ChemBioChem 9: 406-412.
Gocke D, Nguyen CL, Pohl M, Stillger T, Walter L, Müller M. 2007. Branched-chain keto acid decarboxylase from Lactococcus lactis (KdcA), a valuable thiamine diphosphate-dependent enzyme for asymmetric C-C bond formation. Adv. Synth. Catal. 349: 1425-1435.
Berthold CL, Gocke D, Wood MD, Leeper FJ, Pohl M, Schneider G. 2007. Crystal structure of the branched-chain keto acid decarboxylase (KdcA) from Lactococcus lactis provides insights into the structural basis for the chemo- and enantioselective carboligation reaction. Acta Crystallogr. Sec. D: Biol. Crystallogr. 63: 1217-1224.
Spaepen S, Versées W, Gocke D, Pohl M, Steyaert J, Vanderleyden J. 2007. Characterization of phenylpyruvate decarboxylase, involved in auxin production of Azospirillum brasilense. J. Bacteriol. 189 (21): 7626-7633.
Domínguez de María P, Pohl M, Gocke D, Gröger H, Trauthwein H, Walter L, Müller M. 2007. Asymmetric synthesis of aliphatic 2-hydroxy ketones by enzymatic carboligation of aldehydes. Eur. J. Org. Chem.: 2940-2944.
Hischer T, Gocke D, Fernandez M, Hoyos P, Alcantara AR, Sinisterra JV, Hartmeier W, Ansorge-Schumacher MB. 2005. Stereoselective synthesis of novel benzoins catalysed by benzaldehyde lyase in a gel-stabilised two-phase system. Tetrahedron 61(31): 7378-7383.
monographs
Macedo de MeloE, ClaassenC, FinniganW, de SouzaR O M A, Rother D. Multi-enzyme catalysed processes in asymmetric synthesis: state-of-the-art and future trends, from: De Gonzalo and Alcántara - Biocatalysis in Asymmetric Synthesis, 2024 Elsevier, Oxford, 371-402
Sehl T, Kulig J, Westphal R, Rother D. 2015. Synthetic enzyme cascades for valuable diols and amino alcohols – smart composition and optimization strategies. Grunwald P, editor. Industrial Biocatalysis. Pan Stanford Publishing Pte. Ltd., Singapore, ISBN 978-981-4463-88-1 (Hardcover), 978-981-4463-89-8 (eBook)
Pohl M, Gocke D, Müller M. 2008. Thiamin-based Enzymes for Biotransformations. Anastas PT, Crabtree RH, editors. Handbook of Green Chemistry – Green Catalysis. Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA. S. 75-114.
Gocke D. 2008. New and optimised thiamine diphosphate (ThDP)-dependent enzymes for carboligation - Creation of a toolbox for chiral 2-hydroxy ketones. Institute of Molecular Enzyme Technology, Heinrich-Heine-University Düsseldorf, PhD-thesis.
patents
Sehl T, Baraibar A G, Pohl M, Rother D. Verfahren zur Herstellung von Cathin.
PCT/EP patent 3008198, patent granted: 10.05.2017
US-patent 9,890,406, patent granted: 13.02.2018
US-patent 9,890,406, patent granted: 13.02.2018
Japanese patent 6427176, granted: 02.11.2018
Indian patent 317326, granted: 31.07.2019
Chinese patent ZL201480032090.0, granted: 26.03.2019
Sehl T, Marx L, Westphal R, Pohl M, Rother D. Lyase und für die DNA kodierende DNA, die DNA enthaltenden Vektoren sowie Verfahren zur asymmetrischen Synthese von (S)-Phenylacetylcarbinol.
US-patent 10,006,061, patent granted: 26.06.2018
PCT/EP patent 3194587, patent granted: 14.08.2019
Japanese-patent 6802167, patent granted: 30.11.2020
Chinese Patent ZL201580049907.X, patent granted: 29.10.2021
Sehl T, Marx L, Westphal R, Pohl M, Rother D. Lyase sowie Verfahren zur asymmetrischen Synthese von (S)-Phenylacetylcarbinol.
US-patent 10,465,211, patent granted: 05.11.2019
PCT/EP patent 3194588, patent granted: 21.10.2020
García Ovejero J, Marmenia I, Veintemillas Verdaguer s, Martínes de la Fuente J, Morelase Herrero M P, Grazú Bonavia M V, Guisán Sejas J M, López Gallego F, Nidetzky B, Rother D, Cassinelli N, Bernadini G B. Nanoparticles for the control of one-spot multi-enzyme eratcions.
File No priority declaration: EP21382585, patent filed: 01.07.2021
Last Modified: 14.10.2025