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Theoretical Physics of Living Matter Seminar: 
From single cells to emergent mechanical properties of tissue 


Anfang
26.11.2020 11:00 Uhr
Veranstaltungsort
Remote access

Oliver Otto

Zentrum für Innovationskompetenz: Humorale Immunreaktionen in kardiovaskulären Erkrankungen,

Universität Greifswald, Greifswald, Germany

Join us at: www.fz-juelich.de/ibi/TPLMSem

The mechanical properties of cells have long been established as a sensitive and label-free biomarker for cell and tissue function. While mechanical cell assays have traditionally been limited to low throughput or small sample size, our development of real-time deformability cytometry increased analysis rates to up to 1,000 cells per second.

Theoretical Physics

In my presentation I will discuss how this technology provided a new perspective on life science research by enabling the first time a high-throughput mechanical characterization of heterogeneous biological samples on a single cell level. This includes monitoring the invasion of the Malaria parasite into erythrocytes, studying the impact of magnetic nanoparticles on blood platelets, and to assess the quality of manufactured red blood cells. The second part of my presentation focuses on multicellular structures and the question how mechanical properties of cells contribute to tissue rheology.

Until recently, a high-throughput technology for mechanical characterization of tissue had been elusive. We answered this challenge by introducing virtual fluidic channels. Virtual channels are liquid-bound microfluidic constrictions of variable cross-sections, which can be modified within seconds and tailored to hydrodynamic stress distributions to match a wide size range of biological samples.

We perform high-throughput rheology on spheroids as a 3D tissue model system and demonstrate that the Young’s modulus of isolated cells exceeds the one of tissue by one order of magnitude. Interestingly, our results suggest that tissue elasticity is mainly driven by cell-cell interactions and extracellular matrix contributions.


Kontakt

Dr. Dmitry Fedosov
Telefon: +49 2461 61-2972
Fax: +49 2461 61-3180
E-Mail: d.fedosov@fz-juelich.de