Navigation and service

Advertising division: ICS-4 - Cellular Biophysics
Reference number: 2019D-185

test

At the Institute of Complex Systems - Cellular Biophysics (ICS-4), biologists, chemists, physicians and physicists are working together to understand cellular signal processing and its alteration in human disease. The focus is on ion channels, ion transporters and receptors that allow cells to respond to extracellular signals and communicate with other cells.
The main goal of the project is to elucidate the functional role of chloride/proton exchangers in transmitter filling and exocytosis of synaptic vesicles. Efficient recycling of synaptic vesicles at the presynaptic nerve terminal is essential to maintain synaptic transmission. After endocytosis, retrieved vesicles have to be re-acidified, refilled with neurotransmitter and positioned correctly at the release site for a second round of exocytosis. We are interested in identify the exact vesicle trafficking step where chloride/proton exchangers exert its function and how they regulate acidification and neurotransmitter filling. We are using primary cell cultures of hippocampal neurons and chromaffin cells from different genetically modify mouse lines and a combination of patch-clamp and optical electrophysiology.

We are offering a

PhD Position in the field of neurophysiology with a focus on the function of intracellular chloride/proton exchangers in exocytosis

Your Job:

  • Characterization of the exocytotic responses of chromaffin cells from Clcn3-/-, Clcn4-/- or Clcn5-/- as well as double mutant Clcn3-/-/Clcn4-/- mice
  • Implementation of knock-down strategy to reduce the expression of ClC-5 and generate a the triple mutant condition
  • Measurement of exocytosis using patch-clamp and estimate the catecholamine content using carbon fiber amperometric

Your Profile:

  • University degree (Master) in Biology, or related natural sciences disciplines (Biomedicine, Pharmacy or Molecular Medicine)
  • Strong interest in electrophysiology
  • Prior experience in patch-clamp recordings and primary cell cultures are appreciated, but not prerequisite
  • High degree of responsibility, and ability to work in a team in different environments
  • Good communication and written skills in English

Our Offer:

  • A multidisciplinary scientific environment with outstanding technical infrastructure – ideal conditions for successfully completing the suggested project for a doctoral degree
  • Opportunity to get expertise in state-of art techniques and strategies in the field of patch-clamp and optical electrophysiological methods to study exocytosis and recycling of intracellular organelles
  • Beeing part of the DFG-funded project “Functional role of intracellular chloride/proton exchangers ClC-3, ClC-4 and ClC-5 in neurosecretion”
  • A contract for the duration of 3 years
  • We will provide continuous scientific mentoring and discussions by the scientist directing this project
  • The doctoral degree will be conferred by the Heinrich Heine University, Düsseldorf
  • Further development of your personal strengths, e.g. via a comprehensive further training programme
  • Pay in line with 50 % of pay group 13 of the Collective Agreement for the Public Service (TVöD-Bund)


Forschungszentrum Jülich aims to employ more women in this area and therefore particularly welcomes applications from women.

We also welcome applications from disabled persons.

Further information on the institute is available at:
https://www.fz-juelich.de/ics/ics-4/EN/Home/home_node.html

Additional Information

We look forward to receiving your application, preferably online via our online recruitment system on our career site until 07.10.2019, quoting the above-mentioned reference number.

Questions about the vacancy?
Contact us by mentioning the reference number 2019D-185: career@fz-juelich.de
Please note that for technical reasons we cannot accept applications via email.