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Advertising division: IBG-1 - Biotechnology
Reference number: 2019M-092, Biotechnology

Bachelor or Master thesis: Light-regulated synthetic enzyme cascades

Our group:
The ‘Biocatalysis’ group focusses on the optimization of enzymes and biocatalytic processes for the production of preferably chiral fine chemicals. Therefore, we use an integrated engineering approach to tailor enzymes and reactions systems for a desired application and create enzyme toolboxes. Besides multi-step syntheses with purified enzymes, the potential of synthetic enzyme cascades using whole cell biocatalysts in aqueous as well as in non-conventional media is investigated.

Project description:
In multi-step enzyme cascades cross-reactivity can occur (1), when enzymes are combined in a one-pot reaction system, which diminishes the yield of the final product. To avoid cross-reactivity, the target enzymes will be inactivated by light after the respective reaction is completed. The goal of this project is to characterize fusion enzymes, optimize light-inactivation setups and establish a light-regulated multi-step cascade towards the pharmaceutical product tetrahydroisoquinoline.

Your qualifications:
You are a bachelor or master student from the field of biotechnology. Experiences regarding microbial cultivations, enzymatic assays and HPLC analysis are beneficial.

If you are interested and if you would like to find out more about this project, please send your application, CV and current transcript of records via Email to:
Tim Gerlach
Forschungszentrum Jülich
Institute of Bio- and Geosciences
IBG-1: Biotechnology

Announcement as pdf file:  Bachelor or Master thesis: Light-regulated synthetic enzyme cascades (PDF, 218 kB)